VIPSolutionsAdecapeptide undergoes partial hydrolysis to give peptides whose amino acid compositions are shown. Reaction of the intact decapeptide with Edman's reagent releases PTH-Val. What is the sequence of the decapeptide? 1.lle, Phe 2 le, Avg Aa 34g lle le, ser 5 Pe, le Glu 6.6i Val 7 Ser lle Tre 8.His, Arg. Tre, Ser Spall out the name of the polypeptide using three leter codes for each amino acid separated with hyphens (for example, TyrLys-Trp).
Question:
Adecapeptide undergoes partial hydrolysis to give peptides whose amino acid compositions are shown. Reaction of the intact decapeptide with Edman's reagent releases PTH-Val. What is the sequence of the decapeptide?
1.lle, Phe
2 le, Avg Aa
34g lle
le, ser
5 Pe, le Glu
6.6i Val
7 Ser lle Tre
8.His, Arg. Tre, Ser
Spall out the name of the polypeptide using three leter codes for each amino acid separated with hyphens (for example, TyrLys-Trp).
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Asked by: Test User 100005
Created at: 2025-05-12 08:50:27
Asked by: Test User 100005
Created at: 2025-05-12 08:50:27
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Let's break down the problem step-by-step.
### **Given:**
- **Partial hydrolysis** gave us the following fragments:
1. Ile, Phe
2. Ile, Arg, Ala
3. Arg, Ile
4. Ile, Ser
5. Phe, Ile, Glu
6. Glu, Val
7. Ser, Ile, Tre
8. His, Arg, Tre, Ser
- **Edman degradation** releases **PTH-Val**, meaning **Val** is at the **N-terminus**.
### **Process:**
Let's try to reconstruct the sequence:
#### 1. **Start with Val at the N-terminus.**
#### 2. **Look for overlaps:**
Fragment 6: **Glu, Val**
→ Val must be followed by Glu.
**Sequence so far: Val-Glu-...**
Fragment 5: **Phe, Ile, Glu**
→ Glu is in the middle, so it could be ...-Glu-Phe-Ile... or ...-Phe-Ile-Glu...
Fragment 1: **Ile, Phe**
→ Phe is before Ile.
Fragment 2: **Ile, Arg, Ala**
Fragment 3: **Arg, Ile**
Fragment 4: **Ile, Ser**
Fragment 7: **Ser, Ile, Tre**
Fragment 8: **His, Arg, Tre, Ser**
Let's try to chain them:
- From above, Val-Glu-...-Phe-Ile-...
- Phe is before Ile, so: Val-Glu-Phe-Ile-...
Now let's try to fit the rest:
- Fragment 2: **Ile, Arg, Ala**
After Ile, we have Arg and Ala.
So: Val-Glu-Phe-Ile-Arg-Ala
- Fragment 3: **Arg, Ile**
Arg is before Ile, but we've already used Ile after Phe, then Arg, then Ala. So this fits.
- Fragment 4: **Ile, Ser**
After Ile, Ser comes next.
- Fragment 7: **Ser, Ile, Tre**
After Ser, Ile, then Tre.
- Fragment 8: **His, Arg, Tre, Ser**
This means before Arg, we have His, and after Arg, Tre and Ser.
Let's try to put it all together:
1. **Val-Glu-Phe-Ile-Arg-Ala-Ser-Ile-Thr-His**
But let's double-check with all fragments:
- Val-Glu (**fragment 6**)
- Glu-Phe-Ile (**fragment 5**)
- Ile, Phe (**fragment 1**)
- Ile-Arg-Ala (**fragment 2**)
- Arg-Ile (**fragment 3**)
- Ile-Ser (**fragment 4**)
- Ser-Ile-Thr (**fragment 7**)
- His-Arg-Thr-Ser (**fragment 8**)
Now, given the fragment **His-Arg-Thr-Ser**, it seems **His** comes before **Arg**, and after Arg, it's Thr and Ser.
So, let's adjust:
- After **Ala**, maybe comes **His**, then **Arg**, then **Thr**, then **Ser**.
So, the most likely sequence is:
**Val-Glu-Phe-Ile-Ala-His-Arg-Thr-Ser-Ile**
### **Final Polypeptide Sequence (three-letter codes, hyphen separated):**
**Val-Glu-Phe-Ile-Ala-His-Arg-Thr-Ser-Ile**
---
**If you need the one-letter code:**
**V-E-F-I-A-H-R-T-S-I**
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