Give step-by-step explanation and final answer Chinese hamster ovary
(
CHO
)
cells are grown in a stirred tank of diameter
2
m and liquid volume
0
.
9
m
^
(
3
)
.
The stirrer speed is
5
rpm
.
The vessel has four
1
0
%
baffles and is sparged with air at a flow rate of
0
.
5
vvm
(
volume of gas per volume of liquid per minute
)
.
A protectant chemical is added to the medium to protect the cells against the effects of bursting bubbles. The density of the medium is
1
0
0
0
kg
/
m
^
(
3
)
and the viscosity is
1
.
2
cP
.
The impeller is a Rushton turbine of diameter
0
.
8
m
.
a
)
Is the air dispersed effectively in this system? b
)
Is the flow turbulent? c
)
What proportion of the power input is from sparging? d
)
What should be the air flow rate so that mixing can operate at the flooding
-
loading transition?
Question:
Give step-by-step explanation and final answer Chinese hamster ovary
(
CHO
)
cells are grown in a stirred tank of diameter
2
m and liquid volume
0
.
9
m
^
(
3
)
.
The stirrer speed is
5
rpm
.
The vessel has four
1
0
%
baffles and is sparged with air at a flow rate of
0
.
5
vvm
(
volume of gas per volume of liquid per minute
)
.
A protectant chemical is added to the medium to protect the cells against the effects of bursting bubbles. The density of the medium is
1
0
0
0
kg
/
m
^
(
3
)
and the viscosity is
1
.
2
cP
.
The impeller is a Rushton turbine of diameter
0
.
8
m
.
a
)
Is the air dispersed effectively in this system? b
)
Is the flow turbulent? c
)
What proportion of the power input is from sparging? d
)
What should be the air flow rate so that mixing can operate at the flooding
-
loading transition?
This is AI generated Response.
Asked by: Test User 100192
Created at: 2025-05-17 21:41:34
Final Answer:
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Let's tackle each part step by step, with equations and explanations:
---
### **Given Data**
- **Tank diameter (\(D_T\))**: 2 m
- **Liquid volume (\(V_L\))**: 0.9 m³
- **Stirrer speed (\(N\))**: 5 rpm = 5/60 = 0.0833 s⁻¹
- **Baffles**: Four, each 10% of tank diameter
- **Air flow rate (\(Q_{air}\))**: 0.5 vvm
- **Density (\(\rho\))**: 1000 kg/m³
- **Viscosity (\(\mu\))**: 1.2 cP = 1.2 × 10⁻³ Pa·s
- **Impeller diameter (\(D\))**: 0.8 m
- **Impeller type**: Rushton turbine
---
## **a) Is the air dispersed effectively in this system?**
**Effective air dispersion** occurs if the **power input from the impeller is sufficient to disperse the air bubbles** (i.e., not in the "flooded" regime).
We use the **flooding-loading transition** criterion, usually described by the **critical superficial gas velocity (\(u_{gc}\))** or the ratio \(Q_{air}/Q_{critical}\).
### **Step 1: Calculate air flow rate in m³/s**
\[
Q_{air} = 0.5 \times V_L \text{ per minute} = 0.5 \times 0.9 = 0.45 \text{ m}^3/\text{min}
\]
\[
Q_{air} = 0.45/60 = 0.0075 \text{ m}^3/\text{s}
\]
---
### **Step 2: Calculate superficial gas velocity (\(u_g\))**
\[
u_g = \frac{Q_{air}}{A}
\]
Where \(A\) is the cross-sectional area of the tank:
\[
A = \frac{\pi D_T^2}{4} = \frac{\pi \times 2^2}{4} = \pi \text{ m}^2 \approx 3.142 \text{ m}^2
\]
\[
u_g = \frac{0.0075}{3.142} = 0.00239 \text{ m/s}
\]
---
### **Step 3: Calculate impeller power input (ungassed)**
For a **Rushton turbine**, the **power number (\(N_P\))** ≈ 5 (for turbulent regime).
\[
P = N_P \cdot \rho N^3 D^5
\]
\[
N = 0.0833 \text{ s}^{-1},\ D = 0.8 \text{ m}
\]
\[
P = 5 \times 1000 \times (0.0833)^3 \times (0.8)^5
\]
Calculate step-by-step:
- \(N^3 = (0.0833)^3 = 0.000578\)
- \(D^5 = (0.8)^5 = 0.32768\)
- \(P = 5 \times 1000 \times 0.000578 \times 0.32768\)
- \(5 \times 1000 = 5000\)
- \(0.000578 \times 0.32768 = 0.0001897\)
- \(5000 \times 0.0001897 = 0.9485\ \text{W}\)
\[
P \approx 0.95\ \text{W}
\]
**This is a low value**—in reality, this is because the stirrer speed is very low for a tank of this size. But let's proceed.
---
### **Step 4: Compare to air flow (flooding criterion)**
The **flooding velocity** for a Rushton turbine is (van’t Riet, 1979):
\[
Q_{flood} = K \cdot N D^3
\]
Where \(K\) ≈ 0.2 for Rushton turbine.
\[
Q_{flood} = 0.2 \times 0.0833 \times (0.8)^3 = 0.2 \times 0.0833 \times 0.512 = 0.2 \times 0.0427 = 0.00854\ \text{m}^3/\text{s}
\]
Our actual \(Q_{air} = 0.0075\ \text{m}^3/\text{s}\) < \(Q_{flood} = 0.00854\ \text{m}^3/\text{s}\)
**Therefore, the air flow is just below the flooding limit.**
- **Air is (just) being dispersed effectively, but very close to flooding.**
---
## **b) Is the flow turbulent?**
Calculate the **Reynolds number (\(Re\))** for the impeller.
\[
Re = \frac{\rho N D^2}{\mu}
\]
Given:
- \(\rho = 1000\ \text{kg/m}^3\)
- \(N = 0.0833\ \text{s}^{-1}\)
- \(D = 0.8\ \text{m}\)
- \(\mu = 1.2 \times 10^{-3}\ \text{Pa·s}\)
\[
Re = \frac{1000 \times 0.0833 \times 0.8^2}{1.2 \times 10^{-3}}
\]
\[
0.8^2 = 0.64
\]
\[
1000 \times 0.0833 \times 0.64 = 53.312
\]
\[
Re = \frac{53.312}{1.2 \times 10^{-3}} = 44,427
\]
**Since \(Re \gg 10,000\), the flow is highly turbulent.**
---
## **c) What proportion of the power input is from sparging?**
**Power input from sparging (gas):**
\[
P_{gas} = Q_{air} \cdot \rho \cdot g \cdot h
\]
Where:
- \(Q_{air} = 0.0075\ \text{m}^3/\text{s}\)
- \(\rho = 1.2\ \text{kg/m}^3\) (for air!),
- \(g = 9.81\ \text{m/s}^2\)
- \(h\) = liquid height (\(V_L/A = 0.9/3.142 = 0.286\ \text{m}\))
\[
P_{gas} = 0.0075 \times 1.2 \times 9.81 \times 0.286
\]
\[
1.2 \times 9.81 = 11.77
\]
\[
11.77 \times 0.286 = 3.369
\]
\[
0.0075 \times 3.369 = 0.0252\ \text{W}
\]
**Impeller power = 0.95 W** (as above).
**Total input = 0.95 + 0.025 = 0.975 W**
\[
\text{Fraction from sparging} = \frac{0.025}{0.975} \approx 2.6\%
\]
---
## **d) What should be the air flow rate for mixing at the flooding-loading transition?**
Use the **flooding flow rate** calculated above:
\[
Q_{flood} = 0.00854\ \text{m}^3/\text{s}
\]
Convert to vvm:
\[
Q_{flood,\ min} = 0.00854\ \text{m}^3/\text{s} \times 60 = 0.512\ \text{m}^3/\text{min}
\]
\[
\text{vvm} = \frac{0.512}{0.9} = 0.569\ \text{vvm}
\]
---
## **Summary Table**
| Part | Answer |
|---|---|
| a) | **Yes, air is just being dispersed effectively (Q_air is slightly below flooding limit).** |
| b) | **Flow is highly turbulent (Re ≈ 44,400).** |
| c) | **Proportion of power from sparging is ≈ 2.6%.** |
| d) | **Flooding-loading transition occurs at Q_air = 0.00854 m³/s (0.569 vvm).** |
---
### **Final Answers**
**a)** Yes, air is being effectively dispersed, since the air flow is just below the flooding limit.
**b)** The flow is highly turbulent (Re = 44,400).
**c)** About 2.6% of the total power input is from sparging.
**d)** The air flow rate for operation at the flooding-loading transition should be 0.00854 m³/s, which is 0.569 vvm.
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